The role of 1-deoxy-d-xylulose-5-phosphate synthase and phytoene synthase gene family in citrus carotenoid accumulation.
Identifieur interne : 000C90 ( Main/Exploration ); précédent : 000C89; suivant : 000C91The role of 1-deoxy-d-xylulose-5-phosphate synthase and phytoene synthase gene family in citrus carotenoid accumulation.
Auteurs : Gang Peng [République populaire de Chine] ; Chunyan Wang ; Song Song ; Xiumin Fu ; Muhammad Azam ; Don Grierson ; Changjie XuSource :
- Plant physiology and biochemistry : PPB [ 1873-2690 ] ; 2013.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Carotenoids, Geranylgeranyl-Diphosphate Geranylgeranyltransferase, Transferases.
- enzymology : Citrus.
- metabolism : Citrus.
Abstract
Three 1-deoxy-D-xylulose-5-phosphate synthases (DXS) and three phytoene synthases (PSY) were identified in citrus, from Affymetrix GeneChip Citrus Genome Array, GenBank and public orange genome databases. Tissue-specific expression analysis of these genes was carried out on fruit peel and flesh, flower and leaf of Satsuma mandarin (Citrus unshiu Marc.) in order to determine their roles in carotenoid accumulation in different tissues. Expression of CitDXS1 and CitPSY1 was highest in all test tissues, while that of CitDXS2 and CitPSY2 was lower, and that of CitDXS3 and CitPSY3 undetectable. The transcript profiles of CitDXS1 and CitPSY1 paralleled carotenoid accumulation in flesh of Satsuma mandarin and orange (Citrus sinensis Osbeck) during fruit development, and CitPSY1 expression was also associated with carotenoid accumulation in peel, while the CitDXS1 transcript level was only weakly correlated with carotenoid accumulation in peel. Similar results were obtained following correlation analysis between expression of CitDXS1 and CitPSY1 and carotenoid accumulation in peel and flesh of 16 citrus cultivars. These findings identify CitPSY1 and CitDXS1 as the main gene members controlling carotenoid biosynthesis in citrus fruit. Furthermore, chromoplasts were extracted from flesh tissue of these citrus, and chromoplasts of different shape (spindle or globular), different size, and color depth were observed in different cultivars, indicating chromoplast abundance, number per gram tissue, size and color depth were closely correlated with carotenoid content in most cultivars. The relationship between carotenoid biosynthesis and chromoplast development was discussed.
DOI: 10.1016/j.plaphy.2013.06.031
PubMed: 23883976
Affiliations:
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Electronic address: steven19831204@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Wang, Chunyan" sort="Wang, Chunyan" uniqKey="Wang C" first="Chunyan" last="Wang">Chunyan Wang</name></author><author><name sortKey="Song, Song" sort="Song, Song" uniqKey="Song S" first="Song" last="Song">Song Song</name></author><author><name sortKey="Fu, Xiumin" sort="Fu, Xiumin" uniqKey="Fu X" first="Xiumin" last="Fu">Xiumin Fu</name></author><author><name sortKey="Azam, Muhammad" sort="Azam, Muhammad" uniqKey="Azam M" first="Muhammad" last="Azam">Muhammad Azam</name></author><author><name sortKey="Grierson, Don" sort="Grierson, Don" uniqKey="Grierson D" first="Don" last="Grierson">Don Grierson</name></author><author><name sortKey="Xu, Changjie" sort="Xu, Changjie" uniqKey="Xu C" first="Changjie" last="Xu">Changjie Xu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23883976</idno><idno type="pmid">23883976</idno><idno type="doi">10.1016/j.plaphy.2013.06.031</idno><idno type="wicri:Area/PubMed/Corpus">000433</idno><idno type="wicri:Area/PubMed/Curation">000433</idno><idno type="wicri:Area/PubMed/Checkpoint">000433</idno><idno type="wicri:Area/Ncbi/Merge">001359</idno><idno type="wicri:Area/Ncbi/Curation">001359</idno><idno type="wicri:Area/Ncbi/Checkpoint">001359</idno><idno type="wicri:Area/Main/Merge">000C94</idno><idno type="wicri:Area/Main/Curation">000C90</idno><idno type="wicri:Area/Main/Exploration">000C90</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The role of 1-deoxy-d-xylulose-5-phosphate synthase and phytoene synthase gene family in citrus carotenoid accumulation.</title><author><name sortKey="Peng, Gang" sort="Peng, Gang" uniqKey="Peng G" first="Gang" last="Peng">Gang Peng</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. Electronic address: steven19831204@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Wang, Chunyan" sort="Wang, Chunyan" uniqKey="Wang C" first="Chunyan" last="Wang">Chunyan Wang</name></author><author><name sortKey="Song, Song" sort="Song, Song" uniqKey="Song S" first="Song" last="Song">Song Song</name></author><author><name sortKey="Fu, Xiumin" sort="Fu, Xiumin" uniqKey="Fu X" first="Xiumin" last="Fu">Xiumin Fu</name></author><author><name sortKey="Azam, Muhammad" sort="Azam, Muhammad" uniqKey="Azam M" first="Muhammad" last="Azam">Muhammad Azam</name></author><author><name sortKey="Grierson, Don" sort="Grierson, Don" uniqKey="Grierson D" first="Don" last="Grierson">Don Grierson</name></author><author><name sortKey="Xu, Changjie" sort="Xu, Changjie" uniqKey="Xu C" first="Changjie" last="Xu">Changjie Xu</name></author></analytic><series><title level="j">Plant physiology and biochemistry : PPB</title><idno type="eISSN">1873-2690</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carotenoids (metabolism)</term><term>Citrus (enzymology)</term><term>Citrus (metabolism)</term><term>Geranylgeranyl-Diphosphate Geranylgeranyltransferase (metabolism)</term><term>Transferases (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carotenoids</term><term>Geranylgeranyl-Diphosphate Geranylgeranyltransferase</term><term>Transferases</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Three 1-deoxy-D-xylulose-5-phosphate synthases (DXS) and three phytoene synthases (PSY) were identified in citrus, from Affymetrix GeneChip Citrus Genome Array, GenBank and public orange genome databases. Tissue-specific expression analysis of these genes was carried out on fruit peel and flesh, flower and leaf of Satsuma mandarin (Citrus unshiu Marc.) in order to determine their roles in carotenoid accumulation in different tissues. Expression of CitDXS1 and CitPSY1 was highest in all test tissues, while that of CitDXS2 and CitPSY2 was lower, and that of CitDXS3 and CitPSY3 undetectable. The transcript profiles of CitDXS1 and CitPSY1 paralleled carotenoid accumulation in flesh of Satsuma mandarin and orange (Citrus sinensis Osbeck) during fruit development, and CitPSY1 expression was also associated with carotenoid accumulation in peel, while the CitDXS1 transcript level was only weakly correlated with carotenoid accumulation in peel. Similar results were obtained following correlation analysis between expression of CitDXS1 and CitPSY1 and carotenoid accumulation in peel and flesh of 16 citrus cultivars. These findings identify CitPSY1 and CitDXS1 as the main gene members controlling carotenoid biosynthesis in citrus fruit. Furthermore, chromoplasts were extracted from flesh tissue of these citrus, and chromoplasts of different shape (spindle or globular), different size, and color depth were observed in different cultivars, indicating chromoplast abundance, number per gram tissue, size and color depth were closely correlated with carotenoid content in most cultivars. The relationship between carotenoid biosynthesis and chromoplast development was discussed.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement><orgName><li>Université de Zhejiang</li></orgName></list><tree><noCountry><name sortKey="Azam, Muhammad" sort="Azam, Muhammad" uniqKey="Azam M" first="Muhammad" last="Azam">Muhammad Azam</name><name sortKey="Fu, Xiumin" sort="Fu, Xiumin" uniqKey="Fu X" first="Xiumin" last="Fu">Xiumin Fu</name><name sortKey="Grierson, Don" sort="Grierson, Don" uniqKey="Grierson D" first="Don" last="Grierson">Don Grierson</name><name sortKey="Song, Song" sort="Song, Song" uniqKey="Song S" first="Song" last="Song">Song Song</name><name sortKey="Wang, Chunyan" sort="Wang, Chunyan" uniqKey="Wang C" first="Chunyan" last="Wang">Chunyan Wang</name><name sortKey="Xu, Changjie" sort="Xu, Changjie" uniqKey="Xu C" first="Changjie" last="Xu">Changjie Xu</name></noCountry><country name="République populaire de Chine"><region name="Zhejiang"><name sortKey="Peng, Gang" sort="Peng, Gang" uniqKey="Peng G" first="Gang" last="Peng">Gang Peng</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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